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Low serum trough levels are associated with post-surgical recurrence in Crohn's disease patients undergoing prophylaxis with adalimumab
Digestive and Liver Disease, Volume 46, Issue 11, November 2014, Pages 1043–1046
Whether therapeutic drug monitoring of biologic therapy can predict the efficacy of adalimumab to prevent postoperative Crohn's disease recurrence is unknown.
To investigate whether adalimumab trough levels and anti-adalimumab antibodies correlate with endoscopic and clinical outcomes in a series of patients treated with prophylactic adalimumab monotherapy after resective surgery.
Post hoc analysis of a randomized, mesalamine-controlled trial. Adalimumab trough levels and antibodies were analysed every 8 weeks for 2 years using an homogeneous mobility shift assay.
At two years, 1/6 patient had clinical recurrence and 1/6 patient had endoscopic and clinical recurrence. At baseline (9.5 vs. 14.4 mcg/mL) and during follow-up [7.5 (4.4–9.8) vs. 13.9 (8.9–23.6) mcg/mL,p < 0.01], median adalimumab trough levels in patients with clinical or endoscopic recurrence were lower than in those who maintained remission. Persistent antibodies-against-adalimumab were detected in the patient with both endoscopic and clinical recurrence.
Measurement of adalimumab trough levels and anti-adalimumab antibodies after surgery could be useful to further reduce postoperative recurrence.
Keywords: Adalimumab, Anti-tumour necrosis factor alpha antibodies, Crohn's disease, Post-surgical recurrence.
Crohn's disease (CD) is a chronic, disabling and progressive condition. Surgery is not curative and the need for surgery remains high in the biologics era  . Endoscopic postoperative CD recurrence is common and is observed in up to 90% in available reports  . Clinical and surgical recurrence will occur in half and one third of CD patients after a first intestinal resection  . Anti-TNF therapy is the most potent drug class to prevent postoperative CD recurrence, , , , and .
There is growing evidence that therapeutic drug monitoring of anti-TNFα agents (infliximab and adalimumab) and antibodies against anti-TNFα drugs is associated with better outcomes and . Notably, pharmacokinetics of anti-TNF therapy is associated with clinical remission and mucosal healing rates in both CD and ulcerative colitis, , and .
Whether anti-TNF trough levels and antibodies can predict postoperative CD recurrence rates and could be used in the postoperative to guide decision making has yet to be determined.
The aim of this retrospective study was to report a series of patients who underwent intestinal resection for CD and were treated with ADA as prophylactic therapy to prevent CD recurrence for two years. We studied the correlation between trough levels of ADA and the presence of antibodies against ADA (AAAs) with endoscopic and clinical outcomes.
The charts of all ADA-treated CD patients included in our previous randomized, prospective, three-armed, mesalamine-controlled trial with parallel group design, between 2008 and 2010, who had available serial ADA and AAAs levels were screened for inclusion in this post hoc study  . During the trial, patients who underwent resection of macroscopically diseased bowel with complete removal of involved intestine and anastomosis between normal ileum and colon were treated, starting medication within 4 weeks after surgery, with ADA therapy at the induction dose of 160/80 mg, then 40 mg eow for two years. The study protocol was approved by the local independent Ethics Committee and performed according to the Declaration of Helsinki.
After surgery, patients were followed up weekly for the first 4 weeks and then every 8 weeks with detailed history taken, physical examination, blood tests, CD Activity Index (CDAI) and Harvey-Bradshaw Index (HBI) evaluation, and every year, until 2 years after surgery, with endoscopy and magnetic resonance imaging (MRI). In patients who agreed, serum samples for the assessment of ADA and AAAs levels were taken just before each ADA injection at consecutive time points (i.e. every 8 weeks).
Endoscopic recurrence was defined by a score ≥i2 based on Rutgeerts's endoscopic recurrence grading scale. Clinical recurrence was defined as a score of 2 or greater on the clinical recurrence grading scale as proposed by Hanauer. We also evaluated clinical recurrence based on CDAI and HBI for all patients at each time point and recurrence was set in case of a score greater than 200 and 7, respectively, while clinical remission was defined by a CDAI score of less than 150 and a HBI less than or equal to 6. Finally, radiological recurrence was defined as a score of 2 or greater on the radiographic recurrence grading scale.
2.3. Adalimumab trough levels and antidrug antibodies
Adalimumab and AAAs levels in the sera were measured by an homogeneous mobility shift assay [Prometheus Laboratories Inc., San Diego, CA, USA], as previously described  .
Serum trough ADA levels are expressed as μg/ml with the lower limit of quantification for adalimumab being 0.68 mcg/mL. AAAs are expressed in U/mL. The lowest level of quantification for AAAs is 0.55 U/mL, but levels as low as 0.1 U/mL could be detected and used for statistical analysis. Hence all values below 0.1 U/mL were considered undetectable for AAAs.
2.4. Statistical analysis
All data were statistically assessed using SPSS (v17.0). The statistical analysis included descriptive statistics. Presence of AAAs was used as a dichotomous variable: present or absent. For this analysis the Mann–WhitneyUtest was used. Statistical significance was inferred at thep = 0.05 level.
3.1. Characteristics of the six patients at study entry
Six patients (mean age 47, range 34–66) on ADA treatment and with serial blood samples taken for ADA and AAAs measurement were included. The characteristics of these patients are shown in Table 1 . The indication for surgery in these patients was small bowel obstruction (n = 4) and intra-abdominal abscess (n = 2).
|Demographic and clinical features|
|Duration of CD, years||12||18||24||5||5||19|
|Disease Location at surgery||L3||L3||L3||L1||L1||L1|
|Type of surgery||Ileal resection||Ileocecal resection||Ileocecal resection||Ileal resection||Ileocecal resection||Ileocecal resection|
|Reason for surgery||Stenosis||Uncontrolled disease||Stenosis||Stenosis||Uncontrolled disease||Stenosis|
|Prior surgical resection||No||No||No||No||No||No|
|Prior azathioprine use||Yes||Yes||No||No||No||Yes|
|Prior anti-TNFα use||No||No||No||No||No||No|
CD, Crohn's disease; BMI, body mass index; TNFα, tumour necrosis factor alpha.
3.2. Clinical and endoscopic recurrence
As illustrated in Table 2 , at two years of follow-up, 1 patient (P4) had clinical recurrence (CDAI = 180 and HBI = 7), but endoscopic remission (score i1) and one patient (P5) experienced both endoscopic (score i2) and clinical recurrence (CDAI = 290 and HBI = 12). Four patients (P1, P2, P3 and P6) maintained clinical (mean CDAI = 89.5 and HBI = 3) and endoscopic remission (mean score i0) during the whole follow-up period. Mean C-reactive protein (CRP) values were elevated only in the patient who had both clinical and endoscopic recurrence [0.9 (0.4–1.5) mg/dL], whereas CRP of the patient with only clinical recurrence was normal (0.5 (0.2–0.7) mg/dL) as were the values of the other four patients who kept in remission [0.3 (0.1–0.7) mg/dL]. Radiologic recurrence (score = 2) was observed only in the patient (P5) with both endoscopic and clinical recurrence, whereas the remaining patients had no evidence of disease recurrence at MRI assessment.
|Estimates of clinical efficacy|
|At 1 year of follow-up|
|Abnormal median CRP (>0.8 mg/dL)||No||No||No||No||No||No|
|At 2 year of follow-up|
|Abnormal median CRP (>0.8 mg/dL)||No||No||No||No||Yes||No|
|Pharmacokinetics of adalimumab|
|Median ADA levels, mcg/mL||13.0 (9.7–18.6)||13.4 (8.9–17.1)||13.0 (10.7–15.8)||8.3 (6.4–9.8)||6.31 (4.4–9.1)||20.1 (18.5–23.6)|
|AAAs levels at recurrence, U/mL||NA||NA||NA||0.00||0.9||NA|
|ADA levels at recurrence, mcg/mL||NA||NA||NA||7.2||4.4||NA|
CD, Crohn's disease; CDAI, Crohn's disease activity index; HBI, Harvey Bradshaw index; CRP, C reactive protein; ADA, adalimumab; AAA, antibodies against Adalimumab; TNFα, tumour necrosis factor alpha.
3.3. Pharmacokinetics of adalimumab
ADA trough levels at each time point during the follow-up period are illustrated in Fig. 1 . All patients were treated with ADA monotherapy and in those patients with clinical or endoscopic recurrence median ADA trough levels were found to be lower than in those who maintained clinical and endoscopic remission [7.5 (4.4–9.8) mcg/mL vs. 13.9 (8.9–23.6) mcg/mL,p < 0.01]. Interestingly, median ADA trough levels in patients with disease recurrence were lower also at baseline compared to patients who maintained remission (9.5 mcg/mL vs. 14.4 mcg/mL) and progressively decreased over subsequent injections until CD recurrence (P4, from 9.8 mcg/mL to 7.2 mcg/mL and P5, from 9.1 mcg/mL to 4.4 mcg/mL). Moreover, persistent AAAs were determined in the patient with both clinical and endoscopic recurrence (P5), whereas one patient in remission (P1) was found positive for transient AAAs presence throughout the follow-up period. The remaining 4 patients had no evidence of AAAs.
Anti-TNF therapy is increasingly used in the postoperative setting in CD. However, data on the impact of trough serum levels of anti-TNF and anti-drug antibodies in CD patients after intestinal resection on recurrence risk are lacking.
We found that ADA trough levels in patients without recurrence were higher during the follow-up, whereas in patients who had recurrence they progressively decreased until recurrence. These data confirm previous findings that increased ADA trough levels well correlate with the clinical and endoscopic status of CD patients  . However, available studies were retrospective, with blood samples not longitudinally and systematically collected. Furthermore, ADA-treated patients likely had remnant disease in their bowel since they did not undergo resective surgery, a factor that might influence pharmacokinetics of ADA. In contrast, in our study, ADA trough levels as well as AAAs formation could not be influenced by residual disease or systemic inflammation (i.e. alterations in serum albumin levels, TNFα serum and/or tissue concentrations, etc.). Finally, none of our patients took immunosuppressive medications such as azathioprine or methotrexate, which have been recently thought to influence ADA trough levels and AAAs presence  .
Interestingly, we observed that the patient who developed both clinical and endoscopic recurrence had persistent AAAs. We also found that AAAs were absent or at least present in transient form in the remaining patients. These data are in agreement with those recently published by several authors highlighting the importance of antibodies against anti-TNFα drugs in reducing drugs trough levels and favouring the occurrence of loss of response in patients undergoing treatment with either IFX or ADA, , , , and .
It is noteworthy that ADA serum concentrations in our patients who experienced CD recurrence were already low at baseline. This finding could be partly explained by differences in drug clearance between individuals and neutralization of ADA activity by AAAs development  . Moreover, low albumin serum levels were found to be predictive of low values of anti-TNFα serum concentration  . However, in our study, this could not be analysed as all included patients had normal albumin values. Another possible explanation could be difference in body weight of our patients, as increased BMI is a predictor of loss of response in patients on anti-TNF therapy  . Interestingly, both our patients who had recurrence had a BMI higher than 25 kg/m2. These data suggest that low ADA levels at baseline (≤10 mcg/mL) are associated with disease recurrence within 2 years, whereas ADA levels higher than 10 mcg/mL are associated with prolonged remission.
The present observations are limited by the small number of patients included in this post hoc analysis (n = 6), which is at least partly due to the stringent inclusion criteria we applied. The main strengths of our study are the systematic evaluation of clinical, endoscopic and radiologic disease activity in all patients, with a standardized follow-up, and the prospective collection of blood samples at consecutive time points. Furthermore, this is the first investigation aimed to assess the impact of pharmacokinetics of anti-TNF in the postoperative setting.
In conclusion, considering the high costs of biological therapy together with its well-known efficacy at preventing post-surgical recurrence of CD, our preliminary observations suggest that the evaluation of drug trough levels at baseline and during prophylactic treatment after surgery may be useful in order to adopt a tailored use of these drugs and to further decrease CD postoperative recurrence. This may lead to the development of strategies that have a more favourable cost-efficacy ratio in this patient population  . Our findings await confirmation in large prospective studies.
Conflict of interest
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a Division of Gastroenterology, Department of Internal Medicine, University of Genoa, Genoa, Italy
b Inserm U954 and Department of Hepato-Gastroenterology, University Hospital of Nancy, Université de Lorraine, Vandoeuvre-lès-Nancy, France
c Division of Gastroenterology, Department of Surgery, Oncology and Gastroenterology, University of Padua, Padua, Italy
Corresponding author at: Gastroenterology Unit, Department of Surgery, Oncology and Gastroenterology, University of Padua, Via Giustiniani 2, 35128 Padua, Italy. Tel.: +39 049 8217749; fax: +39 049 8760820.
© 2014 Editrice Gastroenterologica Italiana S.r.l., Published by Elsevier B.V.